bruce beutler (Addgene inc)
Addgene inc is a verified supplier 
93
Structured Review
Addgene inc
bruce beutler
Bruce Beutler, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bruce+beutler/pcDNA3-N-HA-NEK7+K64M+(Plasmid+%2375143)/pm39584231-193-69-71
Average 93 stars, based on 3 article reviews
Bruce Beutler, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bruce+beutler/pcDNA3-N-HA-NEK7+K64M+(Plasmid+%2375143)/pm39584231-193-69-71
Average 93 stars, based on 3 article reviews
bruce beutler - by Bioz Stars,
2026-09
93/100 stars
Images
Related Articles
Plasmid Preparation:Article Title: Peli1 facilitates NLRP3 inflammasome activation by mediating ASC ubiquitination. Article Snippet: 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 Bruce Beutler (Addgene plasmid # 75142) pcDNA3-Flag-NLRP3 Nat Immunol. .. 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 Article Title: Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus. Article Snippet: Mutagenesis was performed using the Quikchange I and II Site directed mutagenesis protocols (Agilent Technologies). .. IFNβ luciferase (Addgene)59 pRL-CMV (Promega). pCMV-HA-MyD88 was a gift from Article Title: Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus Article Snippet: Mutagenesis was performed using the Quikchange I and II Site directed mutagenesis protocols (Agilent Technologies). .. IFNβ luciferase (Addgene) pRL-CMV (Promega). pCMV-HA-MyD88 was a gift from Article Title: NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway. Article Snippet: Images were acquired either with a Nikon Eclipse Ti microscope with a 60X Plan Apo Vc objective (numerical aperture, 1.40) and a digital Sight DS-QiMc camera (Nikon) with Z optical spacing of 0.2 μm and 387- nm/447-nm, 472-nm/520-nm, and 650-nm/668-nm filter sets (Semrock) or with Leica TCS SP5 laser scanning microscope mounted on a Leica DMI 6000 CS inverted microscope (Leica Microsystems, Germany). .. Immunoprecipitation. pcDNA3-N-HA-NEK7 was a gift from Article Title: Peli1 facilitates NLRP3 inflammasome activation by mediating ASC ubiquitination. Article Snippet: .. 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 Article Title: Peli1 facilitates NLRP3 inflammasome activation by mediating ASC ubiquitination. Article Snippet: .. 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 Article Title: Peli1 facilitates NLRP3 inflammasome activation by mediating ASC ubiquitination. Article Snippet: Christian Stehlik (Addgene plasmid # 73952) pcDNA3-Flag-ASC Nat Immunol. .. 2016 Mar;17(3):250-8. https://doi.org/10.1038/ni.3333 Article Title: Phosphorylation on serine 72 modulates Rab7A palmitoylation and retromer recruitment. Article Snippet: Materials and Methods Reagents, Cloning and mutagenesis Unless otherwise stated, all reagents used in this study were bought from Fisher Scientific (Ottawa, ON). myc-Rab7A, myc-Rab7AC83,84S, myc-Rab7AC205,207S, RlucII-Rab7A, RlucIIRab7AC83,84S, RlucII-Rab7AC205,207S, sortilin-YFP, Vps26A-GFP10, μ1-GFP10, RILP-GFP10, PLEKHM1-GFP10 and GFP10-FYCO1 were previously described (Modica et al., 2017; Yasa et al., 2020). myc-Rab7AS72A, RlucII-Rab7AS72A, myc-Rab7AY183E, myc-Rab7AY183F, RlucIIRab7AY183E, and RlucII-Rab7AY183F were generated using site generated mutagenesis and verified by sequencing. .. Sortilin-RlucII, CLN6-RlucII, PMP70-RlucII and GFP10-Vps41 were generated by cloning the PCR fragment obtained from Sortilin-YFP (a generous gift from Makoto Kanzaki, Tohoku University), CLN6 (MR219411, Origene Technologies, Inc, Rockville, MD), PMP70-CFP (a generous gift from Frederica Theodoulou, Rothamsted Research) or Vps41 (a generous gift from Jacques Neefjes, Leiden University Medical Center) into pcDNA3.1Hygro(+)GFP10-RlucII-st2 plasmid (a generous gift from Michel Bouvier, Université de Montreal). pcDNA3-N-HA-NEK7 and pcDNA3-N-HA-NEK7K64M were gifts from Luciferase:Article Title: Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus. Article Snippet: Mutagenesis was performed using the Quikchange I and II Site directed mutagenesis protocols (Agilent Technologies). .. IFNβ luciferase (Addgene)59 pRL-CMV (Promega). pCMV-HA-MyD88 was a gift from Article Title: Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus Article Snippet: Mutagenesis was performed using the Quikchange I and II Site directed mutagenesis protocols (Agilent Technologies). .. IFNβ luciferase (Addgene) pRL-CMV (Promega). pCMV-HA-MyD88 was a gift from Immunoprecipitation:Article Title: NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway. Article Snippet: Images were acquired either with a Nikon Eclipse Ti microscope with a 60X Plan Apo Vc objective (numerical aperture, 1.40) and a digital Sight DS-QiMc camera (Nikon) with Z optical spacing of 0.2 μm and 387- nm/447-nm, 472-nm/520-nm, and 650-nm/668-nm filter sets (Semrock) or with Leica TCS SP5 laser scanning microscope mounted on a Leica DMI 6000 CS inverted microscope (Leica Microsystems, Germany). .. Immunoprecipitation. pcDNA3-N-HA-NEK7 was a gift from Generated:Article Title: Phosphorylation on serine 72 modulates Rab7A palmitoylation and retromer recruitment. Article Snippet: Materials and Methods Reagents, Cloning and mutagenesis Unless otherwise stated, all reagents used in this study were bought from Fisher Scientific (Ottawa, ON). myc-Rab7A, myc-Rab7AC83,84S, myc-Rab7AC205,207S, RlucII-Rab7A, RlucIIRab7AC83,84S, RlucII-Rab7AC205,207S, sortilin-YFP, Vps26A-GFP10, μ1-GFP10, RILP-GFP10, PLEKHM1-GFP10 and GFP10-FYCO1 were previously described (Modica et al., 2017; Yasa et al., 2020). myc-Rab7AS72A, RlucII-Rab7AS72A, myc-Rab7AY183E, myc-Rab7AY183F, RlucIIRab7AY183E, and RlucII-Rab7AY183F were generated using site generated mutagenesis and verified by sequencing. .. Sortilin-RlucII, CLN6-RlucII, PMP70-RlucII and GFP10-Vps41 were generated by cloning the PCR fragment obtained from Sortilin-YFP (a generous gift from Makoto Kanzaki, Tohoku University), CLN6 (MR219411, Origene Technologies, Inc, Rockville, MD), PMP70-CFP (a generous gift from Frederica Theodoulou, Rothamsted Research) or Vps41 (a generous gift from Jacques Neefjes, Leiden University Medical Center) into pcDNA3.1Hygro(+)GFP10-RlucII-st2 plasmid (a generous gift from Michel Bouvier, Université de Montreal). pcDNA3-N-HA-NEK7 and pcDNA3-N-HA-NEK7K64M were gifts from Cloning:Article Title: Phosphorylation on serine 72 modulates Rab7A palmitoylation and retromer recruitment. Article Snippet: Materials and Methods Reagents, Cloning and mutagenesis Unless otherwise stated, all reagents used in this study were bought from Fisher Scientific (Ottawa, ON). myc-Rab7A, myc-Rab7AC83,84S, myc-Rab7AC205,207S, RlucII-Rab7A, RlucIIRab7AC83,84S, RlucII-Rab7AC205,207S, sortilin-YFP, Vps26A-GFP10, μ1-GFP10, RILP-GFP10, PLEKHM1-GFP10 and GFP10-FYCO1 were previously described (Modica et al., 2017; Yasa et al., 2020). myc-Rab7AS72A, RlucII-Rab7AS72A, myc-Rab7AY183E, myc-Rab7AY183F, RlucIIRab7AY183E, and RlucII-Rab7AY183F were generated using site generated mutagenesis and verified by sequencing. .. Sortilin-RlucII, CLN6-RlucII, PMP70-RlucII and GFP10-Vps41 were generated by cloning the PCR fragment obtained from Sortilin-YFP (a generous gift from Makoto Kanzaki, Tohoku University), CLN6 (MR219411, Origene Technologies, Inc, Rockville, MD), PMP70-CFP (a generous gift from Frederica Theodoulou, Rothamsted Research) or Vps41 (a generous gift from Jacques Neefjes, Leiden University Medical Center) into pcDNA3.1Hygro(+)GFP10-RlucII-st2 plasmid (a generous gift from Michel Bouvier, Université de Montreal). pcDNA3-N-HA-NEK7 and pcDNA3-N-HA-NEK7K64M were gifts from Polymerase Chain Reaction:Article Title: Phosphorylation on serine 72 modulates Rab7A palmitoylation and retromer recruitment. Article Snippet: Materials and Methods Reagents, Cloning and mutagenesis Unless otherwise stated, all reagents used in this study were bought from Fisher Scientific (Ottawa, ON). myc-Rab7A, myc-Rab7AC83,84S, myc-Rab7AC205,207S, RlucII-Rab7A, RlucIIRab7AC83,84S, RlucII-Rab7AC205,207S, sortilin-YFP, Vps26A-GFP10, μ1-GFP10, RILP-GFP10, PLEKHM1-GFP10 and GFP10-FYCO1 were previously described (Modica et al., 2017; Yasa et al., 2020). myc-Rab7AS72A, RlucII-Rab7AS72A, myc-Rab7AY183E, myc-Rab7AY183F, RlucIIRab7AY183E, and RlucII-Rab7AY183F were generated using site generated mutagenesis and verified by sequencing. .. Sortilin-RlucII, CLN6-RlucII, PMP70-RlucII and GFP10-Vps41 were generated by cloning the PCR fragment obtained from Sortilin-YFP (a generous gift from Makoto Kanzaki, Tohoku University), CLN6 (MR219411, Origene Technologies, Inc, Rockville, MD), PMP70-CFP (a generous gift from Frederica Theodoulou, Rothamsted Research) or Vps41 (a generous gift from Jacques Neefjes, Leiden University Medical Center) into pcDNA3.1Hygro(+)GFP10-RlucII-st2 plasmid (a generous gift from Michel Bouvier, Université de Montreal). pcDNA3-N-HA-NEK7 and pcDNA3-N-HA-NEK7K64M were gifts from |